SrasV1, Main, Exploration, bibRecord, 002985

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.

Identifieur interne : 002985 ( Main/Exploration ); précédent : 002984; suivant : 002986

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.

Auteurs : Hongyuan Shen [Singapour] ; Shou Guo Fang ; Bo Chen ; Guang Chen ; Felicia P L. Tay ; Ding Xiang Liu

Source :

Journal of virological methods [ 1879-0984 ] ; 2009.

RBID : pubmed:19409420

Descripteurs français

English descriptors

KwdEn :
- Animals, Chick Embryo, Chickens, Dengue Virus (genetics), Genes, Reporter, Genetic Therapy, Genetic Vectors, Genomic Instability, Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Infectious bronchitis virus (genetics), Luciferases, Firefly (genetics), Luciferases, Firefly (metabolism), SARS Virus (genetics), Sequence Deletion, Viral Proteins (genetics), Viral Vaccines (genetics), Viral Vaccines (immunology).
MESH :
- chemical , genetics : Green Fluorescent Proteins, Luciferases, Firefly, Viral Proteins, Viral Vaccines.
- genetics : Dengue Virus, Infectious bronchitis virus, SARS Virus.
- chemical , immunology : Viral Vaccines.
- chemical , metabolism : Green Fluorescent Proteins, Luciferases, Firefly.
- Animals, Chick Embryo, Chickens, Genes, Reporter, Genetic Therapy, Genetic Vectors, Genomic Instability, Sequence Deletion.

Abstract

Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.

DOI: 10.1016/j.jviromet.2009.04.023
PubMed: 19409420

Affiliations:

Singapour

Le document en format XML

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<author><name sortKey="Shen, Hongyuan" sort="Shen, Hongyuan" uniqKey="Shen H" first="Hongyuan" last="Shen">Hongyuan Shen</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Molecular and Cell Biology, Proteos, Singapore, Singapore.</nlm:affiliation>
<country xml:lang="fr">Singapour</country>
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<author><name sortKey="Fang, Shou Guo" sort="Fang, Shou Guo" uniqKey="Fang S" first="Shou Guo" last="Fang">Shou Guo Fang</name>
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<author><name sortKey="Chen, Bo" sort="Chen, Bo" uniqKey="Chen B" first="Bo" last="Chen">Bo Chen</name>
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<author><name sortKey="Chen, Guang" sort="Chen, Guang" uniqKey="Chen G" first="Guang" last="Chen">Guang Chen</name>
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<author><name sortKey="Tay, Felicia P L" sort="Tay, Felicia P L" uniqKey="Tay F" first="Felicia P L" last="Tay">Felicia P L. Tay</name>
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<term>Chick Embryo</term>
<term>Chickens</term>
<term>Dengue Virus (genetics)</term>
<term>Genes, Reporter</term>
<term>Genetic Therapy</term>
<term>Genetic Vectors</term>
<term>Genomic Instability</term>
<term>Green Fluorescent Proteins (genetics)</term>
<term>Green Fluorescent Proteins (metabolism)</term>
<term>Infectious bronchitis virus (genetics)</term>
<term>Luciferases, Firefly (genetics)</term>
<term>Luciferases, Firefly (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>Sequence Deletion</term>
<term>Viral Proteins (genetics)</term>
<term>Viral Vaccines (genetics)</term>
<term>Viral Vaccines (immunology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Délétion de séquence</term>
<term>Embryon de poulet</term>
<term>Gènes rapporteurs</term>
<term>Instabilité du génome</term>
<term>Luciférases des lucioles (génétique)</term>
<term>Luciférases des lucioles (métabolisme)</term>
<term>Poulets</term>
<term>Protéines virales (génétique)</term>
<term>Protéines à fluorescence verte (génétique)</term>
<term>Protéines à fluorescence verte (métabolisme)</term>
<term>Thérapie génétique</term>
<term>Vaccins antiviraux (génétique)</term>
<term>Vaccins antiviraux (immunologie)</term>
<term>Vecteurs génétiques</term>
<term>Virus de la bronchite infectieuse (génétique)</term>
<term>Virus de la dengue (génétique)</term>
<term>Virus du SRAS (génétique)</term>
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<term>Luciferases, Firefly</term>
<term>Viral Proteins</term>
<term>Viral Vaccines</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Dengue Virus</term>
<term>Infectious bronchitis virus</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Luciférases des lucioles</term>
<term>Protéines virales</term>
<term>Protéines à fluorescence verte</term>
<term>Vaccins antiviraux</term>
<term>Virus de la bronchite infectieuse</term>
<term>Virus de la dengue</term>
<term>Virus du SRAS</term>
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<term>Protéines à fluorescence verte</term>
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<term>Chick Embryo</term>
<term>Chickens</term>
<term>Genes, Reporter</term>
<term>Genetic Therapy</term>
<term>Genetic Vectors</term>
<term>Genomic Instability</term>
<term>Sequence Deletion</term>
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<term>Délétion de séquence</term>
<term>Embryon de poulet</term>
<term>Gènes rapporteurs</term>
<term>Instabilité du génome</term>
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<front><div type="abstract" xml:lang="en">Manipulation of the coronavirus genome to accommodate and express foreign genes is an attractive approach for gene delivery and vaccine development. By using an infectious cloning system developed recently for the avian coronavirus infectious bronchitis virus (IBV), the enhanced green fluorescent protein (EGFP) gene, the firefly luciferase gene and several host and viral genes (eIF3f, SARS ORF6, Dengue virus 1 core protein gene) were inserted into various positions of the IBV genome, and the effects on gene expression, virus recovery, and stability in cell culture were studied. Selected viruses were also inoculated into chicken embryos for studies of foreign gene expression at different tissue level. The results demonstrated the stability of recombinant viruses depends on the intrinsic properties of the foreign gene itself as well as the position at which the foreign genes were inserted. For unstable viruses, the loss of expression of the inserted genes was found to result from a large deletion of the inserted gene and even IBV backbone sequences. This represents a promising system for development of coronavirus-based gene delivery vectors and vaccines against coronavirus and other viral infections in chicken.</div>
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<name sortKey="Fang, Shou Guo" sort="Fang, Shou Guo" uniqKey="Fang S" first="Shou Guo" last="Fang">Shou Guo Fang</name>
<name sortKey="Liu, Ding Xiang" sort="Liu, Ding Xiang" uniqKey="Liu D" first="Ding Xiang" last="Liu">Ding Xiang Liu</name>
<name sortKey="Tay, Felicia P L" sort="Tay, Felicia P L" uniqKey="Tay F" first="Felicia P L" last="Tay">Felicia P L. Tay</name>
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<country name="Singapour"><noRegion><name sortKey="Shen, Hongyuan" sort="Shen, Hongyuan" uniqKey="Shen H" first="Hongyuan" last="Shen">Hongyuan Shen</name>
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Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.

Towards construction of viral vectors based on avian coronavirus infectious bronchitis virus for gene delivery and vaccine development.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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